The parasitic wasp Diglyphus isaea belongs to the family Eulophidae. It parasitizes Leaf Miner larvae in field and greenhouse crops. The parasitic wasp is an ectoparasitoid, meaning that it lays eggs at the vicinity of the host (second and third instar larvae of the Leaf Miner). It is native to Europe, Northern Africa and Japan, widely distributed throughout the Holarctic Region; also known from Middle East, Orient, Southeast Asia and Australia.
Leaf-mining flies in the genus Liriomyza (Diptera: Agromyzidae) are the most important hosts of Diglyphus isaea. It attacks nine species of leaf miners, including Liriomyza trifolii, L. bryoniae, L. huidobrensis, L. cicerina, L. congesta, L. pusilla, L. pusio, L. sativae and L. strigata.
Leafminers attack a large number of plants, but seems to favor those in the plant families Cucurbitaceae, Leguminosae and Solanaceae. Diglyphus isaea is most efficient in controlling Liriomyza spp. in crops such as tomato, eggplant, ornamentals and also in open field. Other crops known as hosts include bean, pepper, potato, squash, cucumber, beet, pea and watermelon.
Diglyphus isaea is a small wasp (2 mm long) black with a metallic green sheen bearing short antennas. Females are slightly bigger than males and can be recognized by a wide black-colored band on the back pair of legs while males have two black-colored small bands. Eggs are of elongate shape, transparent white-colored and measure 3 x 0,1 mm.
The adult parasitoid performs especially well during the hot conditions of mid-summer (temperatures above 30°C). It can also feed on the body fluids of the leafminer larvae to obtain protein – an essential ingredient of its diet – to maintain egg production. This activity, known as predatory host feeding, kills the host, in addition to mortality inflicted by active parasitization.
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Bio® Diglyphus – The Product
- Diglyphus isaea is packed in a container of 125 cc containing 250-1000 adults.
- If necessary, Bio®Diglyphus can be stored for 24 hours at a temperature of 6-8°C from the minute of arrival until release. The product should never be frozen.
- Bio®Diglyphus is compatible with selective conventional chemicals (according to the list of compatible chemicals provided by BioBee)
- Bio®Diglyphus is applied while walking in between the plants, opening the container’s lid and letting the wasps fly out towards the plants.
- Application of Bio®Diglyphus should be carried out either early in the morning or late in the afternoon, under relatively mild temperatures.
- Bio®Diglyphus is shipped in isolated styrofoam boxes chilled with icepacks. This packaging must be kept intact till it reaches the end-user. When applied, the containers should be taken from the shipment package one by one and the parasitoids should be released immediately.
- The number of wasps at first introduction, as well as following introductions, depends upon the degree of leafminer infestation and the rate of naturally occurring parasitism.
- About 3 weeks following the release of the parasitoids (depending upon temperature), a clear indication of active parasitism is visible. The subsequent established generations of the parasitic wasp are expected to effectively control the leafminer throughout the entire period of the crop.
BioBee Sde Eliyahu Ltd. produces and markets biological products. Production is carried out using innovative techniques under controlled quality assurance standards such as ISO 9001:2008, as well as IOBC’s international standards for mass-production of insects.
All products are tested to meet specification requirements before leaving the factory.
BioBee is not responsible for the outcome of implementation in the field, as it has no control over the method of application, local conditions, treatment/storage of product not according to instructions, etc.